Each kit includes one Cogent Bidentate C18™ column and one Cogent Diamond Hydride™ column. The C18 column is used for the most hydrophobic compounds while the Diamond Hydride is used for all other compounds.
Using the two columns in this kit, our unique Cogent Diamond Hydride™ column for all hydrodphilic and many hydrophobic metabolites and our Cogent Bidentate C18™ for remaining hydrophobic species, you can easily retain all metabolites using LCMS. Using both Aqueous Normal Phase (ANP) and Reverse Phase (RP) with these columns you can separate both positive and negative species and with the incredible precision that silica hydride columns provide, very accurate “metabolomes” can be produced as reported by Rhee et al.
Precise enough for plotting accurate mass v. retention time, you can identify unknown metabolites or differences between healthy and diseases organisms or cells.
The Metabolomics kit also offers a special discount from list price compared to purchasing these columns separately.
- Metabolomics HPLC Column Kit
- MicroSolv Technology Corp
- 43000-05P-2 / 43000-10P-1 / 43000-10P-2 / 43000-15P-2
- 4 um
- 100 Å
- Aqueous Normal Phase and Reverse Phase
- 0.92 ml/gm
- 350 m2/gm
- (DiaxL) 1.0 or 2.1 x 50 to 150 mm