Fig 1: Poly(ADP-ribose) polymerase and glucose-related metabolism are not involved in mitochondrial membrane potential in LLC-PK1 kidney proximal tubule epithelial cells. LLC-PK1 cells on an 24-well plate were treated with 1 mM 3-aminobenzamide (3-AB) in glucose- and serum-free Dulbecco's modified Eagle's medium medium (vehicle) for 30 minutes and then incubated with 10 mM glucose in XF base medium with 4 mM glutamine (control) for 30 minutes. The cells were stained with 20 nM of tetramethylrhodamine, ethyl ester for 30 minutes, washed three times with 500 µl of phosphate buffered saline/0.2% fetal bovine serum three times, and read by a FilterMax F3 multimode microplate reader (Molecular Devices, Sunnyvale, CA, USA) at excitation and emission wavelengths of 549 and 575 nm, respectively. There are no significant differences (n=4 experiments).