SOLO PCR: PCR Preparation and Purification WorkCell from Hudson Robotics, Inc.

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SOLO PCR: PCR Preparation and Purification WorkCell

Description

Many of our customers wish to use the SOLO pipettor to automate the preparation and purification of their PCR reactions. In response, we have developed a SOLOSoft procedure and a corresponding application note to demonstrate how the SOLO can be used to prepare plates that are ready for the thermocycler. This procedure can be run on a standard SOLO, equipped with a shaker nest.

We have also developed a new workcell designed to support both the PCR preparation protocol, and also PCR purification as well. In addition to the SOLO, this system includes two automated vacuum nests to the left of the SOLO’s deck (Hudson’s VaryVac, P/N 550001 and P/N 550002). The SOLO axis that moves the pipette tip has been extended to reach these nests. A PlateCrane EX robot arm is included to fully automate the process by transferring plates between the sample and vacuum nests. The PlateCrane is equipped with five microplate storage stacks for storing plates, tips and samples for running multiple plates.

The system also comes with our SoftLinx software, and includes a customizable procedure for running a typical purification protocol.