This high-performance 2D capillary-to-microflow HPLC system co-developed by Shimadzu and the NIH (National Institutes of Health) performs separation of complex peptide mixtures with a level of sensitivity and resolution that complements present 2D gel electrophoresis.
Features: It is possible to analyze proteins that cannot be analyzed with 2D gel electrophoresis - highly ionic proteins and hydrophobic proteins can be analyzed on-line. Automatic analysis with high resolution and sensitivity is possible for proteins with low expression levels - protein digests can be analyzed automatically by simply placing them into an autosampler. Assay sensitivity enables trace peptide analysis not always detected on 2-D gels. Stable analysis is ensured by on-line sample trapping and automatic trapping and automatic desalination followed by RP HPLC - a sample from the cation exchange column is trapped on 6 mini trap columns (polymer packed reversed-phase columns with a retention power equivalent to C4) for desalination, followed by reversed-phase analysis. This method ensures stable, precise analysis.
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