Description
Method scouting is a process where different mobile phases and column stationary phases are evaluated to determine the best set of separation conditions for a particular sample. Manual solvent preparation, column switching, and method programming are time-consuming steps, so the Nexera method scouting system was developed to streamline the entire process for more efficient method development.
The system is based around 2 LC-30AD pumps, each with a quaternary valve, which allows the analyst to run binary gradients with 16 different solvent pairs. A high-pressure column selection valve holds from 1 to 6 columns, resulting in up to 96 unique separation conditions per sample. Ultra-high speed conditions can be easily transferred to conventional conditions using a transfer program. In addition, scouting is performed automatically when the mobile phase is switched.
The Nexera Method Scouting Solution software has intuitive, user-friendly screens to set the pump and column valve positions, gradient program steps, and it automatically builds the sequence table in seconds. Method Scouting Solution provides the data required for analysis via a graphical user interface, as well as automated controls conforming to laboratory operations configured in the system settings.