Polymerase chain reaction (PCR) is a workhorse technique of molecular biology laboratories in clinical and basic science. DNA thermal cyclers and gradient PCR units are essential in these laboratories. Selecting a good DNA thermal cycler is important. During PCR analysis, the well or tube capacity will determine the number of samples that can run at one time, including different combinations of temperature gradients (if available), MgCl2 concentrations, and primer levels within a single run. Temperature ranges, temperature ramp speeds, and programmability all influence an instrument’s versatility. PCR machines offer up to 384 well blocks, with temperatures reaching 100C. Some thermocyclers offer gradient capability and heated lids for more precise amplification protocols in PCR testing. The advantage of a PCR thermal cycler without gradient capability is low cost and applicability where thermal or concentration gradients are unnecessary.
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- Temperature Range: 4C to 99C
- Capacity: 10 to 100 uL (96-well), 5 to 25 uL (384-well)
- Heated Lid: Inquire
- Gradient Capability: Yes
- Temperature Range: 30C to 80C
- Capacity: 48 (0.2ml) or 30 (0.5ml) Well Plate
- Heated Lid: Yes
- Gradient Capability: Yes
- Temperature Range: 4C to 100C
- Capacity: 24 (0.2ml; compatible with 8 well microtube strips) or 15 (0.5ml) Wells
- Heated Lid: Yes
- Gradient Capability: Inquire
- Temperature Range: 4C to 100C
- Capacity: 48 (0.2ml), 30 (0.5ml), or half a 96 Well Plate
- Heated Lid: Yes
- Gradient Capability: Yes
- Temperature Range: 0 to 100C
- Capacity: 96 or 384 Wells
- Heated Lid: Yes
- Gradient Capability: Yes
- Temperature Range: 30C to 80C
- Capacity: 60 (0.5 ml), 96 (0.2 ml), or 384 Wells
- Heated Lid: Yes
- Gradient Capability: Yes
- Temperature Range: 4C to 100C
- Capacity: 60 (0.5 ml), 96 (0.2 ml), or 384 Wells
- Heated Lid: Yes
- Gradient Capability: Yes
Microbiology International
- Temperature Range: 40C to 100C
- Capacity: 32 samples (5 to 20 uL)
- Heated Lid: Inquire
- Gradient Capability: Inquire
- Temperature Range: 4C to 99C
- Capacity: 96 Wells
- Heated Lid: Yes (60 to 65C, 100 to 115C)
- Gradient Capability: Yes
- Temperature Range: 4C to 99C
- Capacity: 16 x 0.5 ml (or 25 x 0.2 ml)
- Heated Lid: Yes
- Gradient Capability: No
- Temperature Range: 4C to 99C
- Capacity: 20, 25, 30 or 48 Wells
- Heated Lid: Yes (100 to 115C)
- Gradient Capability: Yes (TC-3000G only)
- Temperature Range: 15C to 100C
- Capacity: 8 x 50 ul (thin-wall plastic tubes)
- Heated Lid: N/A
- Gradient Capability: N/A
- Temperature Range: 4C to 98C
- Capacity: 96 x 0.2 ml
- Heated Lid: 100C to 115C (Adjustable in 1C increments) or Off
- Gradient Capability: Inquire
Thermo Fisher Scientific (LPG)
- Temperature Range: 4C to 99.9C
- Capacity: 96 (0.2 mL), 384 (0.03 mL), 2 x 48 (0.2 mL)
- Heated Lid: Inquire
- Gradient Capability: Yes
Thermo Fisher Scientific (LPG)
- Temperature Range: 4C to 99.9C
- Capacity: Inquire
- Heated Lid: Yes
- Gradient Capability: Inquire
- Temperature Range: Inquire
- Capacity: 18 (0.5ml) or 24 (0.2ml) Wells
- Heated Lid: Yes
- Gradient Capability: Yes (12-row gradient)
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