Polymerase chain reaction (PCR) is a workhorse technique of molecular biology laboratories in clinical and basic science. DNA thermal cyclers and gradient PCR units are essential in these laboratories. Selecting a good DNA thermal cycler is important. During PCR analysis, the well or tube capacity will determine the number of samples that can run at one time, including different combinations of temperature gradients (if available), MgCl2 concentrations, and primer levels within a single run. Temperature ranges, temperature ramp speeds, and programmability all influence an instrument’s versatility. PCR machines offer up to 384 well blocks, with temperatures reaching 100C. Some thermocyclers offer gradient capability and heated lids for more precise amplification protocols in PCR testing. The advantage of a PCR thermal cycler without gradient capability is low cost and applicability where thermal or concentration gradients are unnecessary.
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Eppendorf North America
- 1°C to 30 °C
- 96- and 384-Wells
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- Yes
Corning Incorporated
- 4 to 99.9 °C
- 1 x 96 well plate, 12 8 x 0.2ml strip tubes, 96 x 0.2ml tubes
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Corning Incorporated
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- 1 x 96 well plate, 12 8 x 0.2mL strip tubes, 96 x 0.2mL tubes
- 60° to 65°C, 100° to 115°C
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Eppendorf North America
- 4 to 99 °C
- 96 x 0.2 mL PCR tubes, 1 PCR plate 8 x 12 or up to 71 x 0.5 mL PCR tubes
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- Useful PCR Machine
Eppendorf North America
The Mastercycler Nexus Flat Eco is a PCR system that is flexible and can attach to other PCR machines. Even though it doesn't have its own screen for incorporating the protocols, you can connect this ... Read Review - Thermal Cycler with Unique and Flexible FeaturesThe MaxyGene II Thermal Cycler can be configured to work at different temperatures in the block between one and six. This thermal cycler provides improved performance by reducing time and by-steps, ... Read Review
- Thermal Cycler Quality
Eppendorf North America
I used the Mastercycler Nexus X2 Thermal Cycler for the last 10 years for my research. I trust this thermal cycle because it provides highly reproducible results and is always reliable. I used the ... Read Review - MasterCycler for DNA Amplification
Eppendorf North America
Eppendorf’s Flexlid Mastercycler™ is a highly reproducible amplification machine. It is very efficient to run your samples in this thermal cycler. High temperature protocols on this machine do not ... Read Review - Great and Needful Thermal Cycler
Corning Incorporated
This is an ideal multipurpose thermal cycler for research labs. It has very fast speed with some brand-new features, which provide quality performance & also protect our files with a password. It has ... Read Review - Great and Compact PCR Machine, Very Reliable
Eppendorf North America
I used this machine every day to perform regular PCR and Reverse transcription PCR protocols and for incubation of various samples to do restriction digestion by various restriction enzymes. It's ... Read Review - Flexible PCR Machine
Eppendorf North America
Mastercycler Nexus Flat Eco is a PCR system which is flexible and can attach to other PCR machines. It doesn't have its own screen for incorporating the protocols but you can connect this machine with... Read Review - Expensive but Worth It
Eppendorf North America
Was considering the Mastercycler X50 vs a couple other cheaper options. Ultimately went with the X50 and we don't regret it at all. It's super easy to use, and is very easy to maintain. I've always ... Read Review