Gradient PCR Machine

The polymerase chain reaction (PCR) has become such a ubiquitous technology that professionals from a wide variety of disciplines have begun to depend on it. When selecting a PCR thermal cycler there are three key variables to consider: the number of wells available, the temperature gradient range, and programmability. The number of wells available determines the number of combinations of temperature gradients, MgCl2 concentrations and primer levels, which are the three parameters that determine amplification optimization. Within this matrix, the temperature gradient is a key feature in optimization especially when using heterologous primers. The thermocyclers showcased herein typically offer a maximum gradient temperature range 15 – 30° C. Gradient thermal cyclers vary in temperature ramp speed between cycling steps as well as temperature stability and heated lid control. Other specifications include ease-of-use in programmability and the ability to store programs as part of method development for all basic PCR applications, including molecular cloning and sequencing, diagnostics and forensic analysis.

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SureCycler 8800 Thermal Cycler

SureCycler 8800 Thermal Cycler

Agilent Technologies

  • Temperature Range: 4C to 99C
  • Capacity: 10 to 100 uL (96-well), 5 to 25 uL (384-well)
  • Gradient Temperature Range: 30C to 99C
  • Max. Gradient Range: 30C
  • Heated Lid: Inquire
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  • Temperature Range: 0 to 100C
  • Capacity: 96 or 384 Wells
  • Gradient Temperature Range: 0 to 100C
  • Max. Gradient Range: 30C
  • Heated Lid: Yes
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  • Temperature Range: 4C to 99C
  • Capacity: 96 Wells
  • Gradient Temperature Range: 30C to 99C
  • Max. Gradient Range: 24C Difference
  • Heated Lid: Yes (60 to 65C, 100 to 115C)
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Techne Endurance TC-3000 DNA Thermal Cycler
  • Temperature Range: 4C to 99C
  • Capacity: 20, 25, 30 or 48 Wells
  • Gradient Temperature Range: 20C to 80C
  • Max. Gradient Range: 15C Difference
  • Heated Lid: Yes (100 to 115C)
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Arktik* Thermal Cycler

Thermo Scientific

  • Temperature Range: 4C to 99.9C
  • Capacity: 96 (0.2 mL), 384 (0.03 mL), 2 x 48 (0.2 mL)
  • Gradient Temperature Range: up to 30C
  • Max. Gradient Range: 30C
  • Heated Lid: Inquire
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  • Agilent Technologies
  • Bibby Scientific Ltd.
  • MIDSCI
  • Thermo Scientific

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