Ultra high-performance liquid chromatography, UHPLC, uses columns packed with porous sub-2-µm particles (usually 1.8 µm) to vastly increase throughput and resolving power compared to conventional 5-µm columns. Pressures can surpass 19,000 psi. Limited solvent consumption, the ability to work under a broader pressure range, and lower band broadening are further enhancements. UHPLC’s 10× reduction in analysis time strengthens its popularity in areas including the pharmaceutical industry, food safety, and the clinical field.
The choice of detectors for use with UHPLC is wide-ranging, including UV-VIS, fluorescence, photodiode array, ELSD, quadrupole, and most recently, refractive index. For flexibility, many instruments offer the ability to run both conventional and UHPLC on one instrument. A final tip: When making the switch from HPLC to UHPLC, resist the temptation to overload the smaller columns.
Discover and compare Ultra High Performance Liquid Chromatographs:
Your search for UHPLC returned 23 results.
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Shimadzu
- UV, PDA, ELSD, Fluorescence, RID, Conductivity
- 0.0001 – 3 mL/min (1.0 – 70 MPa); 3 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
- 70 Mpa
Shimadzu
- UV, PDA, ELSD, Fluorescence, RID, Conductivity
- 0.0001 – 3 mL/min (1.0 – 130 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
- 130 Mpa
Shimadzu
- UV, PDA, ELSD, Fluorescence, RID, Conductivity
- 0.0001 – 3 mL/min (1.0 – 105 MPa); 3 – 5 mL/min (1.0 – 80 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
- 105 Mpa
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
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- Inquire
- 1500 bar (22,000 psi)
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
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- Inquire
- Up to 1500 bar (22,000 psi)
Thermo Fisher Scientific
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- 8 mL/min
- Up to 1500 bar (22,000 psi)
Shimadzu
- UV, PDA, ELSD, Fluorescence
- 0.0001 – 3.0000 mL/min (1.0 – 105 MPa); 3.0001 – 5.0000 mL/min (1.0 – 80 MPa); 5.0001 – 10.0000 mL/min (1.0 – 22 MPa)
- 105 Mpa
Thermo Fisher Scientific
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
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- 8 mL/min
- 103.4 Mpa or 1034 bar or 15000 psi
JASCO
- Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer
- PU-4285-BI/PU-4285-LPG: 50 µL to 2.0 mL/minute
- PU-4285-BI/PU-4285-LPG: 1,300 Bar (130 MPa) / AS-4250: 1,300 Bar (130 MPa)
Thermo Fisher Scientific
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- 8 mL/min
- 103.4 Mpa or 1034 bar or 15000 psi
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