Multi Angle Light Scattering (MALS) detectors are benchtop systems for characterization and quantification of proteins, peptides, macromolecules, and polymers. A MALS detector is a type of light scattering measurement system, which allows for the detection of light scattered by components of a solution into multiple distinct angles. Many times MALS detectors are coupled with a separation system, such as FFF or SEC.
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| Company | Waters | Wyatt Technology | Waters | Wyatt Technology |
| Item | DAWN™ Multi-angle Light Scattering (MALS) Instrument | microDAWN™ Multi-Angle Light Scattering Instrument for UHP-SEC-MALS |
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| Catalog Number | DAWN | microDAWN |
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| Description | The DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar massThe DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass, size, conformation, particle concentration, conjugation (e.g. glycoproteins) or encapsulation (e.g. AAV capsid content). It stands out with exceptional sensitivity and the widest range of molecular weight, size, and concentration of any MALS instrument, paired with a broad selection of configurations and optional modules for enhanced capabilities.
The DAWN is most often used in conjunction with gel permeation chromatography (GPC-MALS), size exclusion chromatography (SEC-MALS) or an EclipseTM, field-flow fractionation instrument (FFF-MALS) and may also be used with ion-exchange chromatography or reverse-phase chromatography. The instrument includes a high-resolution, touch-screen front panel to display data graphs, system health and instrument diagnostics and is accompanied by ASTRA™, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.... Read More | The microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size andThe microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects, the microDAWN is similar to the well-established miniDAWN, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius. The microDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion and produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC. In some cases it may be used with ion-exchange or other types of chromatography.... Read More |
| Type | MALS and optional DLS | MALS and optional DLS |
| Measuring Range | 200 Da to 1000 MDa | 200 Da to 10 Mda; 1MDa polymers |
| Detector(s) | High-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS.
Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength. | High-gain, high dynamic range, static light scattering detectors at 3 angles |
| Light Source | 658 nm laser, programmable 10% - 100% of maximum power.
Optional: 785 nm for fluorescence suppression | 660 nm laser, programmable 10% - 100% of maximum power. |
| Wavelength(s) | 658 nm; 785 nm available for fluorescence reduction (with lower sensitivity than 658 nm) | 660 nm |
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