High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.
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- Type: Preparative
- Detector(s): UV, ELSD, RID, Fluorescence and MS
- Flow Rate: 0.001 to 100 mL/min
- Max. Pressure: 400 bar
- Type: Analytical
- Detector(s): Variable Wavelength Detector; Optional: Refractive Index, Fluorescence, Evaporative Light Scattering Detectors or Single Quadrupole MS
- Flow Rate: 0.2 to 10.0 mL/min
- Max. Pressure: 60 Mpa
- Type: Analytical, Semi-prep, Prep
- Detector(s): UV, ELSD, RI, Fluorescence, Third Party Detectors
- Flow Rate: Inquire
- Max. Pressure: Inquire
- Type: Analytical
- Detector(s): Diode Array (UV-VIS)
- Flow Rate: up to 5 ml/min
- Max. Pressure: up to 1200 bar
- Type: Analytical, Semi-prep
- Detector(s): VWD, MWD, DAD, ELSD, FLD, RID, Third Party Detectors
- Flow Rate: 0.2 to 10.0 mL/min (Recommended)
- Max. Pressure: 60 Mpa
- Type: Analytical, Semi-Preparative
- Detector(s): Inquire
- Flow Rate: 0.01 to 9.99 ml/min (Analytical) or 0.10 to 40.0 ml/min (Semi-Preparative)
- Max. Pressure: Inquire
- Type: Analytical, Semi-Preparative
- Detector(s): RI and Fluorescence Detectors
- Flow Rate: 0.01 to 9.99 ml/min (Analytical) or 0.10 to 40.0 ml/min (Semi-Preparative)
- Max. Pressure: Inquire
- Type: Analytical, Semi-Preparative
- Detector(s): Inquire
- Flow Rate: 0.01 to 9.99 ml/min (Analytical) or 0.10 to 40.0 ml/min (Semi-Preparative)
- Max. Pressure: Inquire
- Type: Analytical, Semi-Prep
- Detector(s): CE 4200 Detector or Cecil Fluorescence Detector.
- Flow Rate: 10 ml/min (50 ml /min for semi-preparative pumps)
- Max. Pressure: 40 MPa (15 MPa for semi-preparative pumps)
- Type: Analytical, Semi-Prep
- Detector(s): CE 4200 Detector or Fluorescence Detector
- Flow Rate: 10 ml/min (50 ml /min for semi-preparative pumps)
- Max. Pressure: 40 MPa (15 MPa for semi-preparative pumps)
- Type: Analytical, Semi-Prep
- Detector(s): CE 4200 Detector or Fluorescence Detector
- Flow Rate: 10 ml/min (50 ml /min for semi-preparative pumps)
- Max. Pressure: 40 MPa (15 MPa for semi-preparative pumps)
- Type: Analytical, Semi-Prep
- Detector(s): CE 4200 Dual Wavelength Detector
- Flow Rate: 10 ml/min (50 ml /min for semi-preparative pumps)
- Max. Pressure: 40 MPa (15 MPa for semi-preparative pumps)
- Type: Analytical, Semi-Prep
- Detector(s): UV-Visible Variable Wavelength Detector
- Flow Rate: 10 ml/min (50 ml /min for semi-preparative pumps)
- Max. Pressure: 40 MPa (15 MPa for semi-preparative pumps)
- Type: Analytical, Semi-Prep
- Detector(s): CE 4700 Differential Refractive Index Detector
- Flow Rate: 10 ml/min (50 ml /min for semi-preparative pumps)
- Max. Pressure: 40 MPa (15 MPa for semi-preparative pumps)
- Type: Analytical
- Detector(s): Inquire
- Flow Rate: 10 ml/min (50 ml /min for semi-preparative pumps)
- Max. Pressure: 40 MPa (15 MPa for semi-preparative pumps)
- Type: Analytical
- Detector(s): CE 4200 Dual Wavelength Detector
- Flow Rate: 10 ml/min
- Max. Pressure: 40 MPa
- Type: Analytical
- Detector(s): CE 4200 Dual Wavelength Detector
- Flow Rate: 10 ml/min
- Max. Pressure: 40 MPa
- Type: Analytical, Semi-Preparative
- Detector(s): Inquire
- Flow Rate: 0.15 to 30 mL/min
- Max. Pressure: 5000 psi (Injection), 4350 or 8700 psi (Pump)
- Type: Analytical, Semi-Preparative, Preparative
- Detector(s): UV/Vis, ELSD, MS, DAD or RI
- Flow Rate: 1.0 to 200 mL/min
- Max. Pressure: 5000 psi (Injection), 3040 or 8700 psi (Pump)
- Type: Preparative
- Detector(s): Microprocessor Controlled Absorbance Detector (Dual, Variable Wavelengths)
- Flow Rate: 1 to 100 mL/min (depending on pump head)
- Max. Pressure: 4060 psi (depending on pump head)
Hitachi High Technologies America, Inc.
- Type: Preparative
- Detector(s): UV-Vis, ELSD and RI
- Flow Rate: 1 to 50 mL/min, 5 to 250 mL/min, or 10 to 500 mL/min
- Max. Pressure: 300 bar
Hitachi High Technologies America, Inc.
- Type: Analytical
- Detector(s): Diode Array, UV, UV-Vis, Fluorescence, RI
- Flow Rate: 0.001 to 9.999 mL/min
- Max. Pressure: 40 MPa (0.001 to 5.000 mL/min) or 20 MPa (5.001 to 9.999 mL/min)
Hitachi High Technologies America, Inc.
- Type: Inquire
- Detector(s): Inquire
- Flow Rate: 0.001 to 10 mL/min
- Max. Pressure: Inquire
Hitachi High Technologies America, Inc.
- Type: Preparative
- Detector(s): UV, UV/Vis, DAD, FL and RI
- Flow Rate: 0.01 to 36.0 mL/min
- Max. Pressure: 6000 psi
Hitachi High Technologies America, Inc.
- Type: Preparative
- Detector(s): UV-Vis, PAD (Pulsed Amperometric Detector) and Fluorescence Detectors
- Flow Rate: Inquire
- Max. Pressure: Inquire
Hitachi High Technologies America, Inc.
- Type: Preparative
- Detector(s): UV, UV-Vis
- Flow Rate: 150 mL/min, 200 mL/min or 250 mL/min
- Max. Pressure: 1500 psi
Hitachi High Technologies America, Inc.
- Type: Analytical
- Detector(s): UV, Diode Array Detector
- Flow Rate: 0.001 to 9.999 mL/min.
- Max. Pressure: 39.2 Mpa
Hitachi High Technologies America, Inc.
- Type: Inquire
- Detector(s): UV, UV-Vis or FL
- Flow Rate: 0.001 to 5.000 mL/min
- Max. Pressure: 60 MPa
- Type: Semi-micro, Analytical, Semi-preparative and Preparative
- Detector(s): UV-Vis, Multi-wavelength, Diode Array, Fluorescence, Circular Dichroism, Chiral, Chemiluminescence, RI Detectors
- Flow Rate: up to 50 ml/min (depending on type)
- Max. Pressure: Inquire
- Type: Preparative Supercritical Fluid Chromatography
- Detector(s): UV-Vis, Multi-channel, CD and more
- Flow Rate: 120 mL/min
- Max. Pressure: Inquire
- Type: Analytical
- Detector(s): Fluorescence, Multi-Wavelength, Circular Dichroism Detectors
- Flow Rate: 0.6 ml/min
- Max. Pressure: up to 15,000 psi
- Type: Analytical
- Detector(s): UV, LCMS
- Flow Rate: Inquire
- Max. Pressure: Inquire
- Type: Analytical
- Detector(s): UV, PDA, Refractive Index, ELSD, LCMS
- Flow Rate: Inquire
- Max. Pressure: Inquire
- Type: Preparative
- Detector(s): UV, LCMS, Refractive Index, ELSD
- Flow Rate: up to 150 ml/min
- Max. Pressure: Inquire
- Type: Analytical
- Detector(s): UV/VIS
- Flow Rate: 0.001 to 5 ml/min
- Max. Pressure: 35 MPa
- Type: Low-Pressure Gradient System
- Detector(s): Inquire
- Flow Rate: 0.08 to 1 mL/min
- Max. Pressure: up to 130 Mpa
- Type: Analytical
- Detector(s): LCMS, ELSD, Conductivity, Refractive Index, UV, PDA, Fluorescence
- Flow Rate: 0.0001 to 5 mL/min
- Max. Pressure: 19,000 psi
- Type: Analytical and Preparative
- Detector(s): LCMS, ELSD-LT, Conductivity, Refractive Index, Spectrophotometric
- Flow Rate: 0.001 to 10 ml/min; 0.01 to 20 ml/min; 0.1 to 150 ml/min
- Max. Pressure: 27.4 MPa / 40 MPa
- Type: Analytical
- Detector(s): UV/VIS, LCMS IT-TOF, ESI-MS
- Flow Rate: 1 nl to 5 µl/min
- Max. Pressure: 20 MPa
- Type: Analytical, Preparative, Semi-Preparative
- Detector(s): Photodiode Array UV-VIS Detector, UV-VIS Detector
- Flow Rate: 0.001 to 150.0 mL/min (depending on model)
- Max. Pressure: up to 49.0 Mpa (depending on model)
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