High-performance liquid chromatography (HPLC) (also called high-pressure liquid chromatography) is a solution-phase technique for fractionation of complex samples. Unlike standard column chromatography, HPLC is an active process in which materials are pumped at high pressure through the separation column, which contains the so-called “stationary phase” of the separation in the form of densely packed, often chemically functionalized beads. Once introduced to the column, it is the sample’s differential affinity for the solvent (“mobile”) and stationary phases that enables separation to occur, generally with sharper resolution than standard liquid chromatography. High performance liquid chromatographs can be used for analytical or preparative applications depending on the HPLC pump and the column size. HPLC columns are available in a variety of formats, including reverse phase, ion exchange, and size exclusion, which separate based on polarity, charge, and size, respectively.
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Other HPLC Instrument Products
JASCO
- Compact HPLC System
- UV-Vis, Fluorescence, PDA, Multi-Wavelength, Circular Dichroism Detectors
- PU-4580: 0.5 uL to 10.0 mL/min
- 50 MPa from 0.5 to 6 mL/min
35MPa up to 10mL/min
Hitachi High-Tech America, Inc.
- Amino Acid Analyzer
- Spectrophotometer: Aberration-corrected, concave diffraction grating
- 0.001 to 1 mL/min
- Up to 34 MPa
JASCO
- Analytical HPLC System
- Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer
- PU-4180: up to 10 mL/min (conventional to semi-prep, up to 10 mm I.D. column)
- 700 Bar (70 MPa)
JASCO
- RHPLC System
- Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer
- PU-4180-LPG: 0.1 mL to 10.0 mL/min
- PU-4285-LPG: 1,300 Bar (130 MPa) / AS-4150: 70 Mpa
JASCO
- Preparative Supercritical Fluid Chromatography (SFC) System
- Standard: PDA / Options: UV-Visible, Chiral (Circular Dichroism), Mass Spectrometer
- PU-4088: 10 to 150 mL/min / PU-4388: 10 to 150 mL/min
- 500 Bar (50 Mpa)
Polymer Char
- Thermal Gradient Interaction Chromatography
- Infrared Detector IR4 and IR5 MCT, Viscometer
- Inquire
- Inquire
SCION Instruments
- HPLC Instrument
- Ultraviolet (UV), Diode Array Detector (DAD), Fluorescence (FL) and Refractive Index (RI)
- Usual: 0.2 to 3.0mL/min
Max.: 10mL/min (solvent: pure water) - 600 Bar quaternary pump with 6-channel degassing and Low Pressure Gradient mixing options
Shimadzu
- Analytical
- UV, LCMS
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Shimadzu
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Shimadzu
- Analytical
- UV/VIS, PDA, RI, Fluorescence, ELSD
- 0.0001 to 10 mL/min
- 70 MPa
Shimadzu
- HPLC
- UV, PDA, ELSD, Fluorescence, RID, Conductivity
- 0.0001 – 5 mL/min (1.0 – 44 MPa); 5 – 10 mL/min (1.0 – 22 MPa)
- 44 Mpa
Shimadzu
- UHPLC
- SPD-M20A
- Inquire
- 100 Mpa
Shimadzu
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Shimadzu
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Thermo Fisher Scientific
- Integrated (U)HPLC system for anayltical purfication by precise fractionation
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- 1500 bar (22,000 psi) depending on configuration
Thermo Fisher Scientific
- Integrated Fraction Collector
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- 0.05 mL/min to 10 mL/min
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Thermo Fisher Scientific
- Dual
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- 8 mL/min
- 103.4 Mpa or 1034 bar or 15000 psi
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
- Automated
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
- Binary
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- 8mL/min
- 103.4 Mpa or 1034 bar or 15000 psi
Thermo Fisher Scientific
- Semi-Preparative
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- 100 mL/min
- 10 MPa (1450 psi)
Thermo Fisher Scientific
- Binary
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
- Quaternary
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- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
- Quaternary
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
- Dual
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- 8 mL/min
- 103.4 Mpa or 1034 bar or 15000 psi
Thermo Fisher Scientific
- Dual
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- 10 mL/min
- 62 Mpa / 620 bar / 9000 psi
Thermo Fisher Scientific
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- 700 bar (10,000 psi)
Thermo Fisher Scientific
- HPLC Instrument
- Choices include: Vanquish Charged Aerosol Detector, Vanquish Diode Array Detector, Vanquish Fluorescence Detector, Vanquish Multiple Wavelength Detector, Vanquish Variable Wavelength Detector, Refractive Index Detector, Mass Spectrometer.
- 0–10 mL/min
- 700 bar
Thermo Fisher Scientific
- HPLC System
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- 700 bar (10,000 psi)
Thermo Fisher Scientific
- UHPLC System
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- 700 bar (10,000 psi)
Thermo Fisher Scientific
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- Up to 1500 bar (22,000 psi)
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Thermo Fisher Scientific
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- 8 mL/min
- Up to 1500 bar (22,000 psi)
Thermo Fisher Scientific
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- 8 mL/min
- up to 1000 bar (15,000psi)
Hitachi High-Tech America, Inc.
- Analytical
- UV Detector, UV-Vis Detector, Diode Array Detector, Fluorescence Detector, RI Detector, MS Detector
- 0.001 to 9.999 mL/min
- up to 60 MPa
Shimadzu
- Inquire
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Shimadzu
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