Microinjection is the process of inserting genetic material into an animal cell or embryo
using a glass micropipet/injection needle to penetrate the cell membrane.
A
microscope with an incorporated micromanipulator for precise needle positioning is used.
Microinjectors are often used in genetic engineering and research, cloning of
organisms, and in cell biology and viral studies.
Clinical applications include in
vitro fertilization and intracytoplasmic sperm injection.
What Are Considerations When Choosing a Microinjection System?
Factors to consider include digital versus manual operation, and constant flow versus
pulsed flow (the latter’s improved precision often reduces damage to the receiving
cell).
Depending on the system, features may include automated microprocessor
control for delicate nanoliter injections, several independent pressure control channels,
and an external pressure supply for injecting volumes greater than 100 pL, such as in
functional genomics.
