Electrospray ionization mass spectrometers (ESI MS) use a so-called “soft” ionization technique that has found widespread use in biological mass spectrometry. Soft ionization techniques enable the ionization of large biological molecules (such as proteins) without fragmenting them, meaning they can be analyzed intact. In an electrospray ionization mass spectrometer, the molecules to be analyzed pass into the mass spectrometer in a fine spray, typically following chromatographic separation, whether via traditional liquid chromatography, HPLC, or nano-LC. As the spray emerges from the ESI source, the molecules are ionized by the nozzle’s electrically charged tip. As this mist travels and evaporates, electrostatic repulsion between like-charged ions ultimately forces the molecules apart. They then may be analyzed by a wide variety of mass analyzers, including quadrupoles, ion traps, time-of-flight, and Fourier transform-based instruments.
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- Mass Analyzer: Quadrupole, TOF, Ion Trap, QQQ or Q-TOF
- Ionization Method(s): ESI, APCI
- Mass Range: m/z 2 to 3000 (depending on model)
- Scanning Speed: up to 5250 u/sec
- Mass Analyzer: Triple Quadrupole
- Ionization Method(s): ESI or APCI
- Mass Range: Inquire
- Scanning Speed: Inquire
- Mass Analyzer: Single Quadrupole
- Ionization Method(s): ESI, APCI, APPI
- Mass Range: Inquire
- Scanning Speed: 10,000 amu/sec (Model 6140)
- Mass Analyzer: Time of Flight
- Ionization Method(s): Electrospray (ESI)
- Mass Range: 20 to 20,000 m/z
- Scanning Speed: up to 40 spectra per sec.
- Mass Analyzer: Ion Trap
- Ionization Method(s): ESI, APCI
- Mass Range: 50 to 4000 m/z
- Scanning Speed: 800 to 27,000 u/s (Depending on model)
- Mass Analyzer: Triple Quadrupole (QqQ)
- Ionization Method(s): ESI, APCI, APPI
- Mass Range: Inquire
- Scanning Speed: Inquire
- Mass Analyzer: Quadrupole Time of Flight (QTOF)
- Ionization Method(s): ESI, APCI, APPI and MALDI
- Mass Range: m/z 20 to 20,000
- Scanning Speed: up to 20 Spectra per sec.
- Mass Analyzer: Ion Trap
- Ionization Method(s): APCI, ESI
- Mass Range: Inquire
- Scanning Speed: 52,000 u/sec
- Mass Analyzer: TOF
- Ionization Method(s): ESI, ESI-nano, APCI, APPI
- Mass Range: 100 to 1000 Da
- Scanning Speed: Inquire
- Mass Analyzer: Qq-TOF
- Ionization Method(s): ESI
- Mass Range: 20 to 40,000 m/z
- Scanning Speed: Inquire
- Mass Analyzer: Single Quadrupole
- Ionization Method(s): ESI, APCI
- Mass Range: 50 to 3000 amu
- Scanning Speed: up to 10,000 amu/sec
Hitachi High Technologies America, Inc.
- Mass Analyzer: Single Quadrupole Analyzer
- Ionization Method(s): Electrospray, ESCi Multimode Ionization, IonSABRE™ APCI, Dual APCI/APPI
- Mass Range: Inquire
- Scanning Speed: up to 10,000 amu/sec
Hitachi High Technologies America, Inc.
- Mass Analyzer: Two high-resolution quadrupole analyzers (MS1/MS2)
- Ionization Method(s): Electrospray, ESCi Multimode Iionization, IonSABRE™ APCI, Dual APCI/APPI
- Mass Range: Inquire
- Scanning Speed: Inquire
- Mass Analyzer: Single Quadrupole
- Ionization Method(s): ESI and/or APCI
- Mass Range: Inquire
- Scanning Speed: 15,000 u/sec
- Mass Analyzer: Molybdenum Hyperbolic Mass Filter with pre-rod
- Ionization Method(s): ESI, APCI, DUIS
- Mass Range: 10 to 2000 m/z
- Scanning Speed: 15,000 u/sec (max)
- Mass Analyzer: MS/MS: Ion Trap/TOF
- Ionization Method(s): APCI or ESI
- Mass Range: Inquire
- Scanning Speed: Inquire
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