As their name implies, ultraviolet visible spectrophotometers (often referred to as a UV Vis spectrophotometer or just a UV spectrophotometer) quantify the optical properties of samples in the ultraviolet and visible wavelength ranges of light (typically 190 to 900/1100 nm). Specifically, UV visible spectroscopy determines how much light of a given wavelength passes through a sample, and how much is absorbed. Such information can be diagnostic of molecular identity, concentration, structure, or activity, whether of organic species such as porphyrins, benzenes, and napthalenes, or of biomolecules like nucleic acids and proteins. Spectrophotometers can scan either fixed (i.e., predefined) or variable wavelengths, and come in both single- and double-beam configurations.
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Berthold Technologies GmbH & Co. KG
- 2048 element CMOS
- 190 to 1,000 nm
- Inquire
- Pulsed Xenon flash lamp
DeNovix Inc.
- Inquire
- 190-840 nm
- 1.5 nm (FWHM at Hg 253.7 nm)
- Pulsed Xenon flash lamp
UV, Blue, Green, Red LEDs
JASCO
- Rowland off-circle arrangement
Single monochromator
Double beam type - 190 to 1,100 nm
- =BS8
- Halogen lamp, Deuterium lamp
JASCO
- Czerny-Turner mount
Single monochromator
Fully symmetrical double beam type - 190 to 2,700 nm (3,200 nm, option)
- =BS11
- Halogen lamp, Deuterium lamp
Agilent Technologies
- Double out-of-plane Littrow monochromator
- 190 to 1100 nm
- 0.1 nm limiting resolution
- Xenon flash lamp
Agilent Technologies
- Double out-of-plane Littrow monochromator
- 190 to 1100 nm
- 0.1 nm limiting resolution
- Xenon flash lamp
Agilent Technologies
- Double out-of-plane Littrow monochromator
- 190 to 1100 nm
- 0.1 nm limiting resolution
- Xenon flash lamp
Agilent Technologies
- Double Beam
- 175 to 900 nm
- Inquire
- Tungsten halogen visible and deuterium arc UV
Agilent Technologies
- Double Beam
- 175 to 3300 nm
- Inquire
- Tungsten halogen visible and deuterium arc UV
Agilent Technologies
- Double beam
- 190 to 1100 nm
- Xenon flash lamp
Agilent Technologies
- Double out-of-plane Littrow monochromator
- 175 to 1800 nm
- UV/Vis less than 0.05; NIR less than 0.1
- Mercury lamp
Agilent Technologies
- Double out-of-plane Littrow monochromator
- 175 to 3300 nm
- Inquire
- Tungsten halogen visible and deuterium arc UV
Analytik Jena
- Double beam spectrophotometer with variable or fixed spectral bandwidth
- 185 - 1200 nm
- Inquire
- Combination of halogen and deuterium lamp. Lamp change can be set to occur between 300 and 450 nm.
DeNovix Inc.
- Inquire
- 190-840 nm
- 1.5 nm (FWHM at Hg 253.7 nm)
- Pulsed Xenon flash lamp
Edinburgh Instruments Ltd
- Czerny-Turner, Dual Beam Monochromator
- 190 to 1100 nm
- Inquire
- Tungsten-Halogen and Deuterium Lamps
JASCO
- Czerny-Turner mount
Double monochromator
Fully symmetrical double beam type - 190 to 900 nm
- =BS9
- Halogen lamp, Deuterium lamp
JASCO
- Czerny-Turner mount
Single monochromator
Fully symmetrical double beam type - 187 to 900 nm
- =BS10
- Halogen lamp, Deuterium lamp
JASCO
- Czerny-Turner mount
Single monochromator
Fully symmetrical double beam type - 190 to 1,600 nm
- =BS12
- Halogen lamp, Deuterium lamp
Mettler-Toledo GmbH
- Inquire
- Inquire
- Inquire
- Inquire
Mettler-Toledo GmbH
- CCD array detector
- 190 to 1,100 nm
- > 1.5 (toluene in hexane abs.)
- Pulsed Xenon flash lamp
Mettler-Toledo GmbH
- CCD array detector
- 190 to 1,100 nm
- > 1.7 (toluene in hexane abs.)
- Pulsed Xenon flash lamp
Mettler-Toledo GmbH
- CCD array detector
- 190 to 1,100 nm
- > 1.9 (toluene in hexane abs.)
- Pulsed Xenon flash lamp
Shimadzu
- Single Beam
- 220 to 800 nm
- +/- 1 nm
- Xenon flash lamp
Shimadzu
- Single-Beam
- 190 to 1,100 nm
- Inquire
- Deuterium (D2) lamp and 20 W halogen (WI) lamp
Shimadzu
- Double beam
- 190 to 1100 nm
- 1 nm
- Inquire
Shimadzu
- Double Beam, Single Monochromator
- 185 to 900 nm or 220 to 1400 nm (when the ISR-2600Plus Integrating Sphere Attachment is used)
- 0.1 nm
- 50 W Halogen Lamp, Deuterium Lamp
Shimadzu
- Double Beam, Double Monochromator
- 185 to 900 nm
- 0.1 nm
- 50 W Halogen Lamp, Deuterium Lamp
Shimadzu
- High-performance double monochromator
- 185 to 3,300 nm
- Maximum resolution: 0.1 nm
- Inquire
Thermo Fisher Scientific
- Dual Beam
- 190 nm to 1100 nm
- Inquire
- Xenon Flash Lamp >5 years typical, 3 years guaranteed)
Thermo Fisher Scientific
- Inquire
- Inquire
- Inquire
- Inquire
Thermo Fisher Scientific
- Dual Beam
- 190 nm to 1100 nm
- Inquire
- Xenon Flash Lamp (>5 years typical, 3 years guaranteed)
Thermo Fisher Scientific
- Double Beam
- 190 nm to 1100 nm
- Inquire
- Xenon Flash Lamp (>5 years typical, 3 years guaranteed)
Thermo Fisher Scientific
- Dual Beam
- 190 nm to 1100 nm
- Inquire
- Xenon Flash Lamp (>5 years typical, 3 years guaranteed)
Thermo Fisher Scientific
- 2048-element CMOS linear image sensor
- 190 to 850 nm
- ≤ 1.8 nm (FWHM at Hg 254 nm)
- Xenon flash lamp
Thermo Fisher Scientific
- 2048-element CMOS Linear Image Sensor
- 260 and 280nm
- ≤ 1.8 nm (FWHM at Hg 254 nm)
- Light emitting diodes
Thermo Fisher Scientific
- 2048-element CMOS Linear Image Sensor
- 190 to 850 nm
- Inquire
- Xenon Flash
Thermo Fisher Scientific
- Inquire
- Inquire
- Inquire
- Inquire
Thermo Fisher Scientific
- 2048-element CMOS Linear Image Sensor
- 190 to 850 nm
- Inquire
- Xenon Flash
Thermo Fisher Scientific
- Double Beam
- 190 to 1100 nm
- Xenon flash lamp
Thermo Fisher Scientific
- Double beam with sample and reference cuvette/accessory positions
- 190 to 1100 nm
- Inquire
- Xenon flash lamp
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- UV-Vis Double Beam Spectrophotometer with Great Accuracy & Scanning Speed
Agilent Technologies
We are using the Cary 3500 UV-Vis double beam type Spectrophotometer for determining the amount of light absorbed or transmitted in different metal and plastic parts. It gives the measurement as a ... Read Review - Spectrophotometry Within Seconds
Mettler-Toledo GmbH
We are using Mettler Toledo Spectrophotometer UV5 in our laboratory and are quite happy with its results so far. It offers a compact system design and easy to use one-click operation to conduct ... Read Review - Single Drop Unit for Easy and Efficient Nucleic Acid Measurements
Thermo Fisher Scientific™
This spectrophotometer is very effective in the quantification of nucleic acid with a single drop of the samples. Very easy to use and have a wi-fi connection with a touch base screen. We have ... Read Review - Quantifying Instrument for Easy Life
Thermo Fisher Scientific™
Very effective, simple to use nano drop to get the highly accurate results for nucleic acid quantification. Straightforward to use and wifi option gives me a cool parameter to transfer the data with ... Read Review - NanoDrop UV-Vis Spectrophotometer
Thermo Fisher Scientific™
Just a simple, small drop (1 µL) has been enough to measure the concentration of DNA, RNA, or protein concentration. It is Easy to manipulate, precise, and fast. The design is beautiful and small and ... Read Review - Nanodrop That Is Not Just for DNA and RNA
Thermo Fisher Scientific™
Just like the previous Nanodrop 2000 version, Nanodrop One is also very impressive for quick and accurate quantification of DNA and RNA. Measures DNA and RNA concentration at ng/uL with 260/280 and ... Read Review - Nanodrop for DNA, RNA and Protein Measurement
Thermo Fisher Scientific™
I used this instrument to quantify and look the quality of DNA, RNA and protein samples. You can detect if there is contamination in your samples. The advantage of this machine is the use of small ... Read Review - Maximize Productivity and Simplify Quantification
Thermo Fisher Scientific™
This next-generation Nanodrop maximizes the productivity of the quantification of various samples by its simple options. It can quantify and provides the quality of the samples by using only 1-2 uL of... Read Review - Marvelous Instrument with Accuracy & Speed
Agilent Technologies
The Universal Measurement Spectrophotometer Cary 7000 model is available in our laboratory and we work with that instrument frequently for measuring optical properties. Sometimes we also use this ... Read Review - It Helps Me to Enhance & Revolutionize Nucleic Acid Research
DeNovix Inc.
In this review, I will delve into my personal experience of working with the DS-11 Series Spectrophotometer / Fluorometer from DeNovix Inc. specifically for nucleic acid analysis applications. This ... Read Review - Getting Fast, Reliable & Precise Results
Berthold Technologies GmbH & Co. KG
The Colibri+ Microvolume Spectrophotometer is very rapid and provides intuitive results of the experiments. In our laboratory, this instrument is used for real-time PCR and sequencing purposes mostly.... Read Review - Determination of DNA/RNA Contamination in Nucleic Acids Made Easy with This Instrument
Thermo Fisher Scientific™
I use this instrument to detect DNA or RNA contamination in nucleic acid samples. By employing microvolume sample measurements, the extracted nucleic acid product is conserved for subsequent testing, ... Read Review - Cary 60 UV-Vis with Great and Reliable Performance
Agilent Technologies
This product is used to measure optical density of bacterial cultures, and is based on the amount of light scattered by the culture. The Cary 60 UV-Vis has a wavelength range of 190-1100 nm, offers ... Read Review - Assessing MSD and ELISA Plates Using the Multiskan SkyHigh Microplate Spectrophotometer
Thermo Scientific
I have used the Multiskan SkyHigh Microplate Spectrophotometer to read absorbance from standard ELISAs and MSD electrochemiluminescence immunoassays. This includes standard curve measurements. The ... Read Review - Amazing Quantification Unit
Thermo Fisher Scientific™
It is very convenient to use in the lab since it can be used by any user, they just have to access their account through their credentials. Multiple machines are not required as we can easily transfer... Read Review - Accurate DS-C Spectrophotometer
DeNovix Inc.
This is a much simpler way of using a classic spectrophotometer. They both have similar results, but this product uses less space in the lab and is much faster to calibrate and use. We use this ... Read Review