As their name implies, ultraviolet visible spectrophotometers (often referred to as a UV Vis spectrophotometer or just a UV spectrophotometer) quantify the optical properties of samples in the ultraviolet and visible wavelength ranges of light (typically 190 to 900/1100 nm). Specifically, UV Vis spectrophotometers determine how much light of a given wavelength passes through a sample, and how much is absorbed. Such information can be diagnostic of molecular identity, concentration, structure, or activity, whether of organic species such as porphyrins, benzenes, and napthalenes, or of biomolecules like nucleic acids and proteins. Spectrophotometers can scan either fixed (i.e., predefined) or variable wavelengths, and come in both single- and double-beam configurations.
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