Spectrofluorometer

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Spectrofluorometers are designed to detect and quantify fluorophores. These are fluorescent molecules that, when exposed to light, absorb photons at a characteristic wavelength. Subsequently, they then emit photons at a different and slightly longer characteristic wavelength. Spectrofluorometers contain excitation and emission filters for using different fluorophores. Monitoring the activities of molecules labeled with fluorophores is crucial to many kinds of experiments in cell biology, molecular biology and genetics, pharmaceuticals, and forensics, among many others. Emitted light may be detected by photomultiplier tubes or photodiodes within the spectrofluorometer, depending on the model. In addition, users of spectrofluorometers now have a wide range of high-tech fluorophores from which to choose to complement the features of a Spectrofluorometer, also referred to as fluorimeter. Modern fluorometers and fluorimeters are capable of detection as low as 1 part per trillion.