Description
ATP-polyamine-biotin, the first cell-permeable ATP analogue, is an efficient kinase cosubstrate. ATP-polyamine-biotin promotes biotin labeling of kinase substrates in live cells.In VitroATP-polyamine-biotin (APB) is incubated with PKA kinase and full-length protein substrate, myelin basic protein (MBP). Biotinylation was observed only in presence of kinase. In addition, MBP biotinylation was lost in the absence of ATP-polyamine-biotin, in presence of the kinase inhibitor staursporine or upon incubation with acid due to cleavage of phosphoramidate bond. Biotinylated kemptide product is observed only in the presence of APB cosubstrate. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:Solid