Description
ATTO488™-dextran is manufactured by reacting a selected dextran fraction with an ATTO488 derivative. The fluorophore ATTO488 displays strong absorption, high quantum yield and photo-stability. After purification from non-bound dye, the products are controlled for molecular weight, solubility, degree of substitution, free dye and loss on drying. Aladdin produce ATTO488-dextran with a molecular weight of 4 kDa.StructureATTO488-dextran is synthesised from well-characterised dextran fractions derived from Leuconostoc mesenteroides via the succinimidyl-derivative of ATTO488. The degree of substitution of ATTO488 is between 0.001-0.008.Spectral dataATTO488-dextran has an absorbance maximum at 502nm (in borate buffer, pH 9.0) and an emission wavelength of 524 nm.ApplicationFluorescent-dextrans are mainly used for studying permeability and microcirculation. Due to the high absorption, high quantum yield and photo-stability, products labeled with ATTO488 can also be used for single-molecule detection, flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and more