Description
Collagenase NB 8 is chromatographically purified; therefore it contains a high collagenolytic activity. The additional enzymatic activities like clostripain, trypsin-like activity and neutral protease, as well as the endotoxin level are reduced.ApplicationCollagenase NB 8 is suitable for dissociation of a broad variety of tissue types.SpecificationsContains class I and class II collagenase (1) as well as reduced levels of clostripain, trypsin-like activities and neutral protease.Chromatographically purified.Activity (Wünsch): ≥ 0.90 U/mgEndotoxin: ≤ 100 EU/mg (Ph. Eur.)(Clostridiopeptidase A)EC 3.4.24.3 • Mr ca. 70 000 - 120 000 (collagenases) • CAS [9001-12-1]References and DefinitionsUnit definition: Collagenase: 1 U according to Wünsch (2) catalyzes the hydrolysis of 1 µmole 4-phenylazobenzyloxycarbonyl-L-prolyl-L-leucylglycyl-L-prolyl-D-arginine per minute at 25 °C, pH 7.1.Endotoxin: Ph. Eur. (1 Endotoxin Unit is equal to 1 International Unit of a WHO approved reference standard endotoxin (RSE)).References1. Bond, M.D. & van Wart, H.E. (1984) Biochemistry 23, 3077-30912. Wünsch, E. & Heidrich, H.G. (1963) Hoppe-Seyler's Z. Physiol. Chem. 333, 149-51