The Tecan SparkCyto plate reader offers real-time detection and analysis of biological, chemical, and physical events, capturing the maximum amount of data from every well, at the same time and under the same conditions. It combines the flexibility of a high-end multimode plate reader with whole well imaging and comprehensive environmental control for cell-based assays. The system uses top-of-the-range camera components and a patent-pending LED autofocus system to provide real-time data acquisition and analysis for 6- to 384-well formats, ensuring that no key event is ever missed. It allows qualitative and quantitative information to be integrated into unique multiparametric data sets, delivering meaningful insights faster than ever. With three magnification levels and four acquisition channels, the plate reader enables entire cell populations to be investigated by capturing the whole well area of 96- or 384-well microplates in just one image, without tiling or distortion.