Shim-pack Bio Diol and IEX columns from Shimadzu Scientific Instruments improve the accuracy of the characterization of peptides, oligonucleotides, and other biopharmaceuticals. Their different pore sizes make columns effective for analysis of aggregates and fragments of mAb, oligonucleotides, and carbohydrates. The columns can reduce particle size from 5 μm to 2 μm to greatly improve the resolution between aggregates and monomers. Users can also reduce the column length from 300 mm to 150 mm using a 2-μm particle to achieve a 50% shorter run time while maintaining resolution. Shim-pack Bio IEX ion exchange columns are available in Q (quaternary ammonium) and SP (sulfopropyl) chemistries and are based on porous (Q and SP columns) and nonporous (Q-NP and SP-NP columns) hydrophilic polymers with low nonspecific adsorption. The porous particles offer good binding capacity with high efficiency, and the nonporous particles offer high efficiency and resolution.