The meteoric rise of cannabis science in recent years has revealed the diverse chemical profiles of today’s cannabis products, including a variety of cannabinoids, terpenes, and other complex organic compounds. As more sophisticated investigations into cannabis compound diversity continue, scientists are expanding their research into the enzymatic processes that are responsible for producing these molecules and the genes that encode them.
Enzymes such as THCA synthase and CBDA synthase are key elements in the folding and final processing of THCA and CBDA. Upregulation of gene expression for these enzymes has given cannabis researchers the ability to direct high-yield production of desired compounds in cannabis products. The medicinal and commercial implications of cannabis genomic analysis is, therefore, paramount to successful operations in the cannabis industry.
Cannabis genomic analysis relies heavily on the usage of genetic material and molecular compounds— meaning ensuring the integrity of your samples is of the utmost importance. Top grade ultra-low freezers are vital in serving as the cannabis researchers’ first line of defense against the considerable drains on resources and project delays resulting from ruined samples due to improper storage conditions.
The lifespan of collection for genetic materials varies depending on the type of low-temperature storage utilized. Low-temperature storage technologies include:
· Refrigeration (+5°C)
· Freeze-Storage
o Freezing (-18°C to -30°C)
o Deep-Freezing (-80°C)
· Cryopreservation (-196°C to -140°C)
o Slow Cooling
o Rapid Cooling
Generally, scientists see an increase in specimen collection lifespans when samples are stored in lower temperature storage technologies. Thus, it is important to purchase reliable ultra-low freezers for long-term storage potential. It is perhaps equally important to make sure your selected ultra-low freezer ensures reliable and efficient storage conditions for your research samples. Make sure you have purchased your ultra-low freezer from reputable manufacturers, such as NuAire, that provide consistently reliable equipment. In addition to the temperature range considerations, storage capacity should be considered when purchasing your ultra-low freezer.
In order to maximize the operating life of your freezer, improve sample viability and reduce sample loss and operating costs, it is imperative to:
· Ensure Proper Maintenance
o Plan for spatial considerations in advance
o Submit a cleaning and maintenance schedule for your lab
o Clear out old samples annually
· Create Sample Organization
o Utilize a label system with an annual review protocol
o Make sure to note anticipated storage times for samples in your inventory
· Develop Emergency Response Planning
o Write out and discuss with your lab personnel a response plan in the event your freezer fails for any reason. Make sure there are alarm systems, fluid contact protocols, and backup space available
· Detail Freezer Management Protocols
o Organize freezer space so that it can be effectively shared
o Do not run freezers that are empty or unused
o Avoid storing materials on top of freezers and try to keep 4” of air circulating on all sides of your freezer
o Ensure that your compressor is running continuously and note strange noises
o Make sure door seals are ensured daily and look for any ice accumulation