Description
This is a method based on Duplex-specific Direct Digestion (DsDD), which overcomes traditional subtraction methods. The Tester and Driver cDNA form drawbacks of hybrids of genes, that are expressed nonspecifically. After hybridization, the hybrid ds cDNA from Tester and Driver cDNA are digested by duplex-specific nuclease. The Driver cDNA is removed by Exonuclease. This method efficiently enriches cDNA specifically expressed in Tester cDNA. Start with established cDNA libraries. Intact recover of subtracted Tester cDNA. 2 day performance.