Description
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.ApplicationHEPES has been used: As a component of homogenisation buffer used in protein sample preparation To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.Other NotesEasily compare specifications for HEPES products with the HEPES specification table