Description
Protein Accession Number: P00330CATH Classification (v. 3.2.0):Class: Alpha BetaArchitecture: Alpha-Beta Complex and 3-Layer (aba) SandwichTopology: Quinone Oxidoreductase; Chain A, domain 1 and Rossmann foldMolecular Weight: 146.8 kDa (Theoretical)Optimal pH: 5.4 (Shore and Theorell 1966)Isoelectric Point: 6.23 (Theoretical)Extinction Coefficient:189,320 cm-1 M-1 (Theoretical)E1%,280 = 12.89 (Theoretical)Activators:Sulfhydryl activating reagentsMercaptoethanolDithiothreitolCysteineHeavy metal chelating agents(White and White 1997)Inhibitors:Heavy metals and -SH reagentsThioureaPurine and pyrimidine derivativesChloroethanol and fluoroethanolN-alkylmaleimidesIodoacetamide1,10-phenanthroline8-hydroxyquinolineBeta-NAD analogs (White and White 1997)Applications:Enzymatic determination of primary alcohols, and aldehydesSynthesis of chiral compoundsSpectrophotometric assay of plasmalogenaseEnzymatic catalysis in organic solventsStudies of NAD+, NADH, NADP+, and NADPH