Description
β-Amyrone (β-Amyron) is a triterpene compound which has anti-inflammatory activity through inhibiting the expression of COX-2. β-Amyrone has antifungal activity, as well as antiviral activity against Chikungunya virus. β-Amyrone also inhibits α-glucosidase and acetylcholinesterase ( AChE ) activity. β-Amyrone can be used in the research of disease like inflammation, infection, and obesityIn Vitroβ-Amyrone (1.25-10 µg/mL, 24 h) inhibits on NO ∙ production in LPS-stimulated J774 cells, with an IC 50 value of 4.61 µg/mL, and has no obvious effect on cell viability. β-Amyrone (10 µg/mL, 24 h) inhibits IL-6, IL-10 levels, and COX-2 expression in LPS-stimulated J774 cells. β-Amyrone (0-235 µM, 7days) has antiviral activity in Vero cells against Chikungunya virus (CHIKV), with EC 50 value of 86 uM. β-Amyrone inhibits α-glucosidase, acetylcholinesterase (AChE) and fungal activity with IC 50 values of 25 µM, 23 µM and 8 µg/mL, respectively. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Western Blot AnalysisCell Line: LPS (1 µg/mL)-stimulated J774 cells Concentration: 2.5, 5, 10 µg/mL Incubation Time: 24 h Result: Inhibited COX-2 expression in a concentration-dependent manner (approximately 90% reduction at 5 or 10 µg/ mL).In Vivoβ-Amyrone (local administration on ear, 0.1-0.6 mg/kg, a single dose) inhibits ear edema formation in phenol-induced edema mice. MCE has not independently confirmed the accuracy of these methods. They are for reference only. Animal Model: Ear phenol-induced edema in Balb C mice Dosage: 0.1, 0.3, 0.6 mg/kg, a single dose Administration: Local administration (20 𝜇L solution) on ear Result: Inhibited ear edema formation in a dose-related manner (47% inhibition at the dose of 0.6mg/kg).IC50& Target:COX-2