Description
β-Estradiol-6-one 6-(O-carboxymethyloxime) is a convenient homogeneous enzyme immunoassay for estradiol is described. Unlike heterogeneous immunoassays, which require time-consuming separation steps or expensive automated systems, homogeneous immunoassays, wherein all reagents are freely suspended in bulk solution, can be simple and fast without costly instrumentation.Application：b-Estradiol-6-one 6-(O-carboxymethyloxime) was shown to exhibit regulatory activities towards the expression of leptin, a protein hormone that coordinate appetite/hunger and metabolism.b-Estradiol-6-one 6-(O-carboxymethyloxime) was shown to control the secretion of leptin via membrane associated estrogen receptor alpha in human placental cells