Description
Extinction CoeffA280 nm = 1.35 at 1.0 mg/mLSpecificityMonospecific for human C2 in human plasma or serumGeneral DescriptionProduct is made from polyclonal antiserum from goats immunized with highly purified human complement protein. Product is the purified IgG fraction. Goats are maintained in FDA certified facilities.Physical Characteristics & StructureAntibodies present are primarily IgG.ApplicationsWestern Blots: Effective at dilutions 1/4,000 to 1/8,000 depending on conditions.Note: Most effective against non-reduced antigen.ELISA: Effective at dilutions 1/8,000 to 1/16,000 depending on conditions.Radial immunodiffusion: 1/44 dilution (Vol/Vol) of anti-C2 IgG fraction in agarose gel results, after staining, in clear precipitin rings with neat, 1:2 and 1:4 dilutions of NHS (3 mm wells, 20 µl sample).versus activated ½ diluted NHS assayed by immunodiffusion and staining of the precipitin band.Immunodiffusion: Effective at 1/8 dilution against NHS or plasma