Description
Calcium chloride is a commonly used biochemical reagent. Calcium plays an important role in many biological processes, such as signaling, muscle contraction, and the maintenance of membrane and cell wall stability. The experimental measurement of biological calcium has been extensively published. Calcium chloride can be used for preparation and transformation of receptive Escherichia coli, and transfection of plasmid DNA or high molecular weight genomic DNA in eukaryotic cells. It has been shown that plasmid DNA pBR322 can be introduced into Escherichia coli by CaCl2 mediated electroporation. The experimental scheme of virus vector concentration by CaCl2 has been published. CaCl2 has been used for stable and two-dimensional crystallization of E. coli derived NADH-ubiquinone REDOX reductase (complex I). The porcine pancreatic elastase crystallized in the presence of CaCl2 and sodium citrate showed that the metal binding site was calcium ion. The effect of CaCl2 on the total fluorescence intensity during the polymerization of E. coli tubulin-like FtsZ splitprotein has been determined by porous fluorescence method