Description
Product description：This product contains 4% SDS, 20% glycerol, 0.02% phenol red and 0.125 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol. SDS-PAGE Sample Buffer is specially formulated for protein sample preparation to based on the Laemmli Method of SDS-PAGE system.Note: Please use after bringing back to normal temperature.Explanation：Preparation of non-reducing sampleA protein sample is mixed with the 2X SDS-PAGE sample buffer (1:1). If necessary, heating the mix solution and then cool down the tube at room temperature. The solution is ready for SDS-PAGE.Preparation of reducing sample (reducing with 2-Mercaptoethanol)Add 10 µL of 2-Mercaptoethanol per 90 µL of 2X SDS-PAGE sample buffer. Mix thoroughly. Then a protein sample is mixed with the sample buffer (1:1) and heating to 95–100°C for 5 min. Cool down the tube at room temperature. The solution is ready for SDS-PAGE