Denhardt′s Solution from Aladdin Scientific Corporation

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This powder can be used to make a 50x concentrate that can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.Denhardt′s Solution has been used:as a component in hybridization buffer for the hybridization of brain sectionsinin-situhybridization peroxidase-(antiperoxidase method-whole mounts) (ISHPAP-WM) processin the preparation of hybridization mixtureSuitable as a blocking Buffer for Southern and Northern blot membranes