Description
Can be used directly for agarose, Ultrodex or polyacrylamide gels and form linear gradients. Carrier Ampholytes ：Ampholytes are low molecular weight molecules of zwitterionic character. They are derived synthetically and comprise a multitude of varying pI-values. In agarose and polyacrylamide gels containing ampholytes, a linear pH-gradient will be built up when an electric field is applied – theampholyte molecules »carry« a net charge and thus migrate in the electric field between the electrodes as long as they will reach the position of corresponding pI. They will stop moving then and form small plateaus (stationary stacks). Benefits of Carrier Ampholytes：high resolution due to multimeric compositionfast staining and destaining timesclear background associated with very lowunspecific binding of dyes and stainshigh solubility in trichloroacetic acid(fast removal of ampholytes during fixation)virtually no interaction with metal ions