Description
Alkaline phosphatase is a broad term associated with non-specific phosphomonoesterases with an alkaline pH optimum.Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.Inibibitors: The enzyme is inhibited by chelating agents and inorganic phosphates.Unit Definitons E. coli. One Unit hydrolyzes 1µmole of p-nitrophenol phosphate per minute at 25°C, pH 8.0