Description
3′-phosphoadenosine 5′-phosphate (PAP), a 3′-phosphorylated nucleotide is found in almost all organisms and is obtained as a by-product of sulfur and lipid metabolism. Purpose Adenosine 3′,5′-diphosphate disodium salt has been used:to spot sample on cellulose high-performance thin-layer chromatography (HPTLC) plates in two-dimensional thin layer chromatographyin enzyme activity assay to study the activities of HOS2/FIERY1 wild typehos2 mutant and fiery1?2 mutant protein against 3′-phosphoadenosine 5′-phosphate (PAP)as a standard for the quantification of phosphoadenosines