Description
MES is one of the so-called 'Good' buffers developed for biological applications. This product is tested for trace ions. A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±-1). Alternatively, volumes of equimolar solutions of MES free acid and MES sodium can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.Application:MES sodium salt has been used:To achieve the required density and stretching of DNA fibers during melting of agarose plugs containing genomic DNATo equilibrate the Balch homogenizer chamber and to prevent sample hydrolysis before sample homogenization