Description
Activity: >400 units/mg protein. One unit is defined as the amount of enzyme which digests hemoglobin releasing peptides which are soluble in 10% TCA. The reaction is measured by an increase of an A280 of 1.0 per 60 minutes at 37ºC.Substrate: acid-denatured hemoglobin (0.2% in reaction mixture).Buffer: 0.1 M formate, pH 3.3