Description
Aladdin IgG Elution Buffer (1x), pH 2.7, is a ready-to-use, amine-based acidic solution designed for the efficient dissociation and recovery of purified antibodies from Protein A, Protein G, Protein A/G, and Protein L affinity supports. This low-pH buffer enables high-yield elution under denaturing conditions and is suitable for a wide range of antibody-antigen or protein-protein affinity purification systems.Key FeaturesHigh-Efficiency Elution: Effectively dissociates bound antibodies from all major IgG-binding proteins under low-pH conditions.Ready-to-Use: Pre-diluted 1× solution requires no preparation—ideal for fast and consistent workflows.Broad Compatibility: Suitable for eluting antibodies in both singleplex and multiplex affinity purification workflows.Consistent Performance: Pre-adjusted to pH 2.7 to ensure reproducible elution profiles across applications.Versatile Application: Also effective in antigen-antibody or general protein-protein interaction studies.ApplicationsAntibody elution in IgG purification using Protein A, G, A/G, or L resinsIdeal for research and development-scale immunoaffinity purificationCompatible with manual and automated chromatography systemsSuitable for elution in most antigen-antibody affinity purification setupsInstructions for UseAfter sample binding and washing steps, apply Aladdin IgG Elution Buffer (1×), pH 2.7, to the affinity resin.Incubate briefly (typically 1-10 minutes) for complete antibody dissociation.Collect eluate fractions and immediately neutralize using an appropriate buffer (e.g., 1 M Tris-HCl, pH 9.0) to preserve antibody activity.For optimal performance, combine with Aladdin’s matching IgG Binding Buffers for a seamless workflow.NotesAvoid prolonged exposure of antibodies to low-pH conditions to prevent denaturation.Neutralize eluted fractions promptly for maximum antibody recovery and stability.For sensitive antibodies, consider Aladdin’s Gentle Ag/Ab Elution Buffer (near-neutral, high-salt)