Description
StableMax RT-qPCR Master Mix (with UDG) is a ready-to-use, all-in-one probe-based master mix for single/multiplex RT-qPCR detection of RNA targets. Optimized buffer simultaneously supports Taq DNA polymerase and reverse transcriptase activity, ensuring broad target compatibility. Pre-mixed working solutions containing primers/probes remain stable at low temperatures without compromising performance. Incorporates dUTP/UDG contamination control to eliminate carryover amplicon contamination. Compatible with standard and fast cycling protocols. Applications:One-step RT-qPCR (RNA targets)Hot-start DNA amplificationKey AdvantagesUnified Buffer System: Optimized for both Taq polymerase and reverse transcriptase.Ready-to-Detect: Add primers/probes to master mix → store as working solution → add sample for direct use.Contamination Control: dUTP/UDG system cleaves uracil-containing contaminants.Broad Compatibility: Adapts to fast cycling (≤40 min) and multiplex assays.Protocol1. Reaction SetupComponentVolume/25 µLVolume/50 µLFinal Conc.StableMax RT-qPCRMaster Mix10 µL20 µL1×Primer-probe Mix1 µL2µL/Templates5 µL10 µL/ddH2OUp to 25 µLUp to 50 µL/Total25 µL50 µL/Notes:Primer/template volumes are adjustable.For fast cycling, use 25 µL total volume.Protect probes from light; avoid freeze-thaw cycles.2. Mixing & CentrifugationGently pipette-mix or vortex lightly.Centrifuge briefly to collect liquid.3. Cycling ConditionsStepTemperature时间循环数150℃15min1295℃2min 30s1394℃10s45455℃40s（collect fluorescence）45Notes1. Reverse transcription (adjust: 5–15 min)2. Initial denaturation (adjust: 1.5–2.5 min)3. Reduce to 40 cycles for high-copy targets.4. Primer Design:Final concentration: 0.2 µMTm ≥55°C (design with tools like OligoAnalyzer™)