MultiScreen-IP Filter Plate, 0.45 µm, clear, sterile MAIPS4510 from MilliporeSigma

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(86) Vaccination with Altered Peptide Ligands of a Plasmodium berghei Circumsporozoite Protein CD8 T-Cell Epitope: A Model to Generate T Cells Resistant to Immune Interference by Polymorphic EpitopesFrontiers in ImmunologyFebruary 14, 2017Gabriela Minigo, Katie L. Flanagan, Robyn M. Slattery, Magdalena Plebanskiwere carried out in 96 well mixed acetate plates (Millipore, Watford, UK) as previously described (8). MAIPS4510 plates, pretreated with 50 μL/well methanol, were used for IFNγ assays; IL-4 and IL-10 secretion was

(87) Alloimmune Monitoring after Islet Transplantation: A Prospective Multicenter Assessment of 25 RecipientsCell TransplantationDecember 1, 2016Vaihere Delaune et al.of 5 days. ALLOIMMUNE MONITORING AFTER ISLET TRANSPLANTATION ELISpot Assessment ELISpot plates (MAIPS4510 Sterile MultiScreen-IP 0.45 mm; Merck Millipore, Schaffhausen, Switzerland) were prepared and coated

(88) Antibody production using a ciliate generates unusual antibody glycoforms displaying enhanced cell-killing activitymAbsNovember 16, 2016Jenny Calow et al.Following digestion, glycans were cleaned using a polyvinylidene fluoride protein-binding membrane plate (MAIPS4510, Millipore). Ion mobility mass spectrometry analysis of released glycans Aqueous solutions (1 μL)

(89) Gel-Trapped Lymphorganogenic Chemokines Trigger Artificial Tertiary Lymphoid Organs and Mount Adaptive Immune Responses In VivoFrontiers in ImmunologyAugust 22, 2016Yuka Kobayashi, Takeshi WatanabeAFC, respectively, as shown previously (50). Hydrophobic PVDF filters on MultiScreenIP Filter Plates (MAIPS4510, Millipore) were coated with 50 μg/ml NP3-BSA, NP30-BSA, or BSA in PBS at 4°C overnight, and then blocked

(90) MRI Based Localisation and Quantification of Abscesses following Experimental S. aureus Intravenous Challenge: Application to Vaccine EvaluationPLOS ONEMay 26, 2016Elizabeth R. Allen, Pauline van Diemen, Yuko Yamaguchi, Claudia Lindemann, Elizabeth Soilleux, Christine Rollier, Fergal Hill, Jurgen Schneider, David H. Wylliepresence of homologous splenocytes (5x106 cells/ml), on High Protein Binding Immobilon-P membrane plates (MAIPS4510, Millipore) coated with 5 mg/ml anti-mouse IFN- γ (AN18, Mabtech). After 18–20 hours incubation, IFN-

MultiScreen-IP Filter Plate, 0.45 µm, clear, sterile