MultiScreen-IP Filter Plate, 0.45 µm, clear, sterile MAIPS4510 from MilliporeSigma

Product Details
ItemMultiScreen-IP Filter Plate, 0.45 µm, clear, sterile
CompanyMilliporeSigma
PriceSupplier Page
Catalog NumberMAIPS4510
Quantity10
ApplicationsCell Culture

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MilliporeSigma

6211 El Camino Real

Carlsbad, CA 92009

United States

Phone: +17607106100

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Website: sigmaaldrich.com/

(146) Dynamic relationship between IFN-gamma and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen loadJournal of immunology (Baltimore, Md. : 1950)April 15, 2007Millington KA, Innes JA, Hackforth S, Hinks TS, Deeks JJ, Dosanjh DP, Guyot-Revol V, Gunatheesan R, Klenerman P, Lalvani A.performed in the same manner as the ex vivo IFN-␥ ELISPOT with the following differences. The membrane of a MAIPS4510 plate (Millipore) was prewet with 50 ␮l of 70% ethanol per well for 2 min at room temperature. Wells

(147) Artificial lymph nodes induce potent secondary immune responses in naive and immunodeficient miceJournal of Clinical InvestigationApril 2, 2007Noriaki Okamoto, Risa Chihara, Chiori Shimizu, Sogo Nishimoto, Takeshi Watanabehighand low-affinity AFCs, respectively). Hydrophobic PVDF filters on MultiScreenIP Filter Plates (MAIPS4510; Millipore) were coated with 50 μg/ml NP3-BSA, NP30-BSA, or BSA in PBS at 4°C overnight, and then blocked with

(148) Comparison of an in-house and a commercial RD1-based ELISPOT-IFN-gamma assay for the diagnosis of Mycobacterium tuberculosis infectionClinical Medicine & ResearchJanuary 11, 2007Paola Mantegani, Federica Piana, Luigi Codecasa, Laura Galli, Paolo Scarpellini, Adriano Lazzarin, Daniela Cirillo, Claudio Fortispreviously described.6 Briefly, the PBMCs (2 x 105 cells/well) were seeded in duplicate in 96-well plates (MAIPS4510, Millipore, Bedford, MA) precoated with an anti-IFN-γ capture monoclonal antibody (mAb) (clone B-B1,

(149) Long-Term Control ofMycobacterium tuberculosisInfection Is Mediated by Dynamic Immune ResponsesThe Journal of ImmunologyJuly 15, 2005Vanja Lazarevic, Dawn Nolt, JoAnne L. Flynnlung and lymph node cells were plated in anti-IFN-␥ Ab-coated (BD Pharmingen; clone R4-6A2) plates (MAIPS4510; Millipore at 80,000 cells/well and 150,000 cell/well, respectively). Cells were incubated in duplicate

(150) Mass Tag-Assisted Identification of Naturally Processed HLA Class II-Presented Meningococcal Peptides Recognized by CD4⁺T LymphocytesThe Journal of ImmunologyMay 1, 2005Hugo D. Meiring et al.ELISPOT assays were performed according to Van Bleek et al. (29). Briefly, 96-well filtration plates (MAIPS4510; Millipore) were coated overnight with anti-IFN-␥ coating Ab (clone 1-D1K; Mabtech) in carbonate buffer

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