MultiScreen-IP Filter Plate, 0.45 µm, clear, sterile MAIPS4510 from MilliporeSigma

Product Details
ItemMultiScreen-IP Filter Plate, 0.45 µm, clear, sterile
CompanyMilliporeSigma
PriceSupplier Page
Catalog NumberMAIPS4510
Quantity10
ApplicationsCell Culture

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MilliporeSigma

6211 El Camino Real

Carlsbad, CA 92009

United States

Phone: +17607106100

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Website: sigmaaldrich.com/

(1) Sequential vaccinations with divergent H1N1 influenza virus strains induce multi-H1 clade neutralizing antibodies in swineNature CommunicationsNovember 27, 2023Kristien Van Reeth, Anna Parys, José Carlos Mancera Gracia, Ivan Trus, Koen Chiers, Philip Meade, Sean Liu, Peter Palese, Florian Krammer, Elien VandoornKitikoon et al.64. Briefly, 96-well ELISpot filter plates (Merck Millipore, Molsheim, France, catalog #MAIPS4510) were coated overnight at 4 °C with 200 HAU per well of live purified IAV. The next day, PBMC were isolated

(2) Intradermal Vaccination against Influenza with a STING-Targeted Nanoparticle Combination Adjuvant Induces Superior Cross-Protective Humoral Immunity in Swine Compared with Intranasal and Intramuscular ImmunizationVaccines (Basel)November 7, 2023Juan F. Hernandez-Franco, Ganesh Yadagiri, Veerupaxagouda Patil, Dina Bugybayeva, Sara Dolatyabi, Ekachai Dumkliang, Mithilesh Singh, Raksha Suresh, Fatema Akter, Jennifer Schrock, et al.utilizing the ELISPOT assay. The day prior to euthanasia, membranes of MultiScreen IP filter plates (MAIPS4510; Millipore Sigma, Burlington, MA, USA) were activated using 20 μL/well of 35% ethanol for 30 sec and

(3) Deletion of the gene for the African swine fever virus BCL-2 family member A179L increases virus uptake and apoptosis but decreases virus spread in macrophages and reduces virulence in pigsJournal of VirologyOctober 31, 2023Ana Luisa Reis, Anusyah Rathakrishnan, Leah V. Goulding, Claire Barber, Lynnette C. Goatley, Linda K. Dixongradient centrifugation. The IFN-γ ELISpot assay has been previously described (65, 66). ELISpot plates (MAIPS4510; Millipore) were coated overnight at 4°C with 4 µg/mL antiporcine IFN-γ mAb P2F6 in 0.05 M carbonate-bicarbonate

(4) Pretransplant BKV-IgG serostatus and BKV-specific ELISPOT assays to predict BKV infection after kidney transplantationFrontiers in ImmunologySeptember 21, 2023Hyunjoo Bae, Seungwon Jung, Byung Ha Chung, Chul Woo Yang, Eun-Jee Ohperformed as described in a previous study (11). Briefly, 96-well ELISPOT plates (Millipore, Cat. No. MAIPS4510) were coated with diluted anti-IFN-γ monoclonal capture antibody at 4°C overnight. After washing the

(5) Generation of antigen-specific memory CD4 T cells by heterologous immunization enhances the magnitude of the germinal center response upon influenza infectionbioRxivSeptember 3, 2023Linda M. Sircy, Andrew G. Ramstead, Hemant Joshi, Andrew Baessler, Ignacio Mena, Adolfo García-Sastre, Matthew A. Williams, J. Scott Scott Halethe Pan B Cell Isolation Kit (Miltenyi Biotec, #130-095-813). MultiScreen-IP Filter Plates (Sigma, #MAIPS4510) were pre-wet with 15 μL 35% ethanol for 30 sec and washed with 1X PBS. Plates were coated with 2 μg/mL

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