Description
The hippocampus is important for memory formation and is severely affected in the brain of Alzheimer's disease (AD). When used in AD models, both APP and the atypical PS1 variant HS showed increased Aβ42/Aβ40 peptide ratio and decreased synaptic protein levels, which are common features of AD. However, these two variants differ in terms of tau hyperphosphorylation, protein aggregation and protein network changes. NeuroD1-mediated gene therapy in HSD-derived progenitor cells can regulate the expression of many genes, including genes related to synaptic transmission. Therefore, HS can be used to reveal the underlying mechanism of early pathogenic changes in the hippocampus of AD patients and provide a powerful platform for the development of treatment strategies for early AD.
Creative Biolabs's Human iPSC-Derived Hippocampal Spheroids are derived from induced pluripotent stem cells (iPSC) without integration under fully defined neural induction conditions.
Creative Biolabs's Human iPSC-Derived Hippocampal Spheroids are rich in hippocampal neurons and express zinc finger and BTB domain-containing protein 20 (ZBTB20) and Prospero homeobox protein 1 (PROX1). In addition, the hippocampal neurons derived from the APP variant HS exhibit profound transcriptome changes, which can be regulated by the overexpression of NeuroD1 (ND1), leading to the upregulation of genes whose gene products are related to synaptic transmission and are altered in AD.