Description
HPAd are prescreened for Adipogenesis Signaling: Primary cultures of adipocytes are prepared by inducing differentiation of human primary preadipocytes and are prescreened for the expression of the following major adipocyte markers:
PPAR_ (Peroxisome proliferator-activated receptor gamma), an adipocyte-specific nuclear hormone receptor
C/EBPα (CCAAT/enhancer binding protein alpha), a transcription factor involved in creating and maintaining the adipocyte phenotype
IR (Insulin receptor), which plays a critical role in induction of adipocyte differentiation, as well as functional regulation
ACCα (Acetyl-CoA carboxylase alpha), a key enzyme for de novo fatty acid synthesis and lipogenic capability of adipocytes
GSK-3β (Glycogen synthase kinase-3-beta), a ubiquitous kinase implicated in regulation of adipocyte gene expression, signaling, adipogenesis, insulin action and glycogen synthesis
Adiponectin, a bioactive adipokine specifically secreted by differentiated adipocyte and involved in metabolic regulation