Description
Magic™ Cell-free Protein Expression in Human Hela Cell Lysate Kit is a mammalian in vitro expression system based on HeLa cell lysates. The kit contains all factors required for protein synthesis including ribosomes, initiation factors, elongation factors and tRNA. Reactions are started by mixing plasmid DNA with the reaction mix. This system can synthesize protein for up to 6 hours.
Magic™ Cell-free Protein Expression in Human Hela Cell Lysate Kit is an optimized kit contains a T7 promoter and an EMCV internal ribosome entry site (IRES) to facilitate high levels of in vitro protein expression. Using a vector containing the EMCV IRES element is critical for obtaining high expression levels in this human in vitro protein expression system.