Description
Rat midbrain neurons (RMbN) are obtained from the midbrain of normal embryonic rats (the 15th day of pregnancy) by a standardized method. When cultured under the recommended conditions, RMbN forms a tree shape and complex formation within one week. The neurite network. RMBN is positive for βIII-tubulin.
The midbrain is a small part of the brain, but plays an important role in the visual and auditory systems, motor control, alertness, sleep/wake and temperature regulation. It contains the substantia nigra, which is a dark area with a large number of dopaminergic neurons, which is closely related to motivation and habits. Midbrain atrophy and diseases seen in diseases such as Parkinson's disease, familial amyotrophic lateral sclerosis, and progressive supranuclear palsy can affect balance, gait, and muscle control. Studying midbrain neurons under those disease conditions will enable people to better understand disease mechanisms, physiopathology, and progress in drug development and treatment. The culture of midbrain neurons can be used in a variety of experiments, including toxicity tests, immunostaining, live cell imaging, co-culture, electrophysiology, etc.