Description
Human adipocytes (HPAd) provide an ideal cell model and facilitate life science research and drug discovery for obesity, diabetes and cardiovascular diseases. We extract HPAd from human subcutaneous and cardiac adipose tissue, and cryopreserve these fibroblast-like precursor cells at the end of the primary culture. They can be transmitted for two passages before differentiating into human adipocytes (HAd). Complete differentiation can be easily achieved by using pre-adipocyte differentiation medium. It is expected that mature HAd should remain healthy 10 days after differentiation induction and should respond within at least 2 weeks after full differentiation. The fat mass can be controlled by inhibiting the differentiation of HPAd and increasing the lipolysis effect. HPAd can also be pre-screened to express adipocyte markers.