Description
Product Characteristics HRP-StabilPLUS is used for the dilution and stabilization of Horseradish Peroxidase (HRP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enables the making of re-diluted, ready-to-use HRP-conjugates, minimizing assay errors in dilution. Superior stabilization of HRP conjugated antibodies in low as well as high protein dilutions is seen, when using HRP-StabilPLUS. Diluted HRPconjugated proteins display stability as follows:• at least 4 years at 2-8 °C • at least 2 years at room temperature • at least 10 weeks at 37 °C.Composition & Properties HRP-StabilPLUS is a ready-to-use buffer that appears as a slightly white solution. The product is based on a mild acid Tris buffer containing proprietary stabilizing components. HRP-StabilPLUS contains neither BSA, nor other material from bovine serum, azide, mercury or other toxic components.Working Procedure 1.Make a series of dilutions of the HRP-conjugated protein in HRP-StabilPLUS in order to determine the optimal dilution. 2.Run the assay as usual or store the diluted HRPconjugated protein preferably at 2-8 °C.Tips & Tricks • HRP-StabilPLUS cannot be used for the dilution of antibodies directed against rabbit immunoglobulins i.e. Swine anti-Rabbit Ig & Goat anti-Rabbit Ig etc. UNI-StabilPLUS (cat. no. U494463) is recommended for this purpose.Handling & Storage • Store solution at 2-8 °C