Description
Pancreatic lipase,when prepared essentially free of colipase,is strongly inhibited by conjugated bile salts at or above their critical micelleconcentration. The addition of colipase restores activity to the bile-salt-inhibited lipase,and gel filtration experiments indicate that colipase in bile salt solution formsa dimer,which createsa 1:1 complex with lipase.PROPERTIESLipase and colipase interact in a stoichiometric relationship, with colipase classified as a co-enzyme for lipase. Colipase is a single-chain polypeptide with a molecular weight of approximately 11,000, containing fivedisulfide bridges and an isoelectric point of 5.0.APPLICATIONUse colipase as a co-emulsifier in diagnostic tests for the determination of lipase activity in combination with a chromogenic lipase substrate.ATTENTIONFor your safety and health, please wear a lab coat and disposable gloves