5-star Goat Polyclonal Anti-tdTomato Antibody

For fixed cell imaging: cells are plated on round coverslips (15 mm) autoclaved or sterilized with ethanol in 12-well dishes to a confluency of 30%–60% Troubleshooting 1a. Cells are plated onto coverslips in the 12-well plate with a volume of 1 mL of medium per well. Let cells attach to coverslips for 24 h prior to experimentation at 37°C in 5% CO2 atmosphere. Cells were fixed with PFA 4%, block cells for 1 h in blocking buffer containing 5% goat serum, 5% bovine serum albumin, PBS, sodium azide to reduce background from nonspecific binding. Goat Polyclonal Anti-tdTomato Antibody was used ON at 1:200 for immunofluorescence.