Staining Cell Membranes Using Wheat Germ Agglutinin (WGA)

I have used the Wheat Germ Agglutinin Alexa Fluor® 488 conjugate (10 μg/ml, Invitrogen) to stain the cell membranes of human chondrocytes. The cells were incubated for 10 minutes in the dark to stain the cell membrane then mounted using Vectashield medium with DAPI for nuclei staining. The cells were imaged using a Leica SP8 confocal 3D Super resolution microscope.