Library Preparation for RNASeq Using TruSeq Stranded mRNA

I have used the TruSeq Stranded mRNA for library preparation for RNA-Seq. Total RNA samples (n=24) were prepared using the ‘TruSeq Stranded mRNA’ kit (Illumina) following manufacturer's instructions, pooled into one library and sequenced on a NextSeq 500 ‘High-Output - 75 cycle’ (Illumina) sequencing run; equivalent to >15 million 75 bp single reads per sample. The data was of publication quality.